Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. All associated StakMax® stackers. We use Spectramax M5 microplate reader in our lab. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. MOLECULAR DEVICES SpectraMax M5 also includes a wide selection of curve-fitting functions, allowing users to perform accurate and reliable quantification of biochemical reactions. analysis, controls the SpectraMax Multi-Mode Microplate Readers. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. SpectraMax® ABS. ® reader to the computer. 1 & 7. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. This application note describes how to. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. A: Microplate Preparation. Materials. 1. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. The spectrophotometric. Most papers they cultured bacteria to OD600>0. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. Pick the best speed, sensitivity, and price for your needs. Expanded wavelength coverage. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. com 13. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. All reagents were brought to room temperature before use. Thank you!. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Table of Contents. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. Download PDF. For a higher . For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Depending on which. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). 0. Table of Contents. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. SpectraMax® Aquamax. com A. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. Our absorbance plate readers feature our PathCheck Sensor technology and. 0 2. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The calculated LOQ values were 0. ATP standard curves read on SpectraMax microplate readers. The CellTiter-Glo assay from Promega uses luciferase. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. 1 2. edu) to be added as a trained user in iLab. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Microplate Readers Biotek Synergy H1 Hybrid Microplate. Turn on the microplate reader. flexibility of monochromator-based systems. Plate chamber design of the SpectraMax category 1 microplate readers. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. e. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Fluorescence Microplate Reader Comparison Chart. In brief, RAW 264. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. Background fluorescence has been subtracted. The cells were then treated to induce apoptosis. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Most papers they cultured bacteria to OD600>0. Optical absorbance. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. Validate the optical performance of your absorbance . Are you using the correct Microplate Adapters? For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. 2. . The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. RAW 264. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. 5 87. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. ,. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. SpectraMax® M4. Supersedes 1. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. 314 low lamp level warning. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email [email protected]% Triton X‐100, 10 mM potassium phosphate buffer pH 7. Figure 1. Add to Cart. When a feature or capability applies to only certain readers, this exception is noted. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. (Shanghai, China). Fluorescence intensity was detected by. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. When a feature or capability applies to only certain readers, this exception is noted. begin reading microplates with seven mouse clicks. 1. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. The advantage of using labeled probes is detection specificity. Wide range of applications The VersaMax reader covers. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. Italic and boldface type are used for emphasis. Note: In this user guide, all references to SpectraMax Multi-Mode. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. FlexStation® 3 Multi-Mode Microplate Reader. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. 26 1. Microplate reader settings. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. SpectraMax® M4. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. The SpectraMax M2 and M2e are versatile and reliable. The SpectraMax® ABS Plus Microplate Reader can run . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Description. Maximum signal with lowest background. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Molecular Devices Spectramax M5 Microplate Reader; Related products. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. SpectraMax® M5e. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Spectramax M5 microplate reader: Molecular Devices: N/A:. SpectraMax Microplate Readers. E Models Only) 69. 6. spr. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Add to Cart. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. 304 signal level saturation. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. 306 plate air cal. The assay window spanned four orders of magnitude. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Other technologies such as imaging. 73. SpectraMax® ABS Plus. The guide also covers the software features, the specifications, and the accessories of the systems. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 6% for the on-board optics (monochromators). • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. After 24 h drug treatment, cells were lysed by RIPA buffer (Life Technologies Inc, US). TR-FRET assay. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 3. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Add to Cart. Microplate Readers Include the M3, M4, M5, and M5. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. TNF standard curve. FlexStation™ II. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Designed to provide the simplicity, flexibility and power required for advanced data analysis. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The cells were incubated with compounds at the indicated doses for 1 h. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 6-384 (96- and 384-well with pipetting) 6-384. 5–100mg/L (ValitaTiter) or 100–2000mg/L (ValitaTiter Plus). Devices’ StakMax® Microplate Stacker in a matter of minutes and . The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. SpectraMax® iD5. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. 1step assay with SpectraMax microplate readers. SpectraMax® ABS. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. 72 MB. 9000-0756. Cuvette port functionality can also be controlled using SoftMax Pro Software. Uncontrolled Copy for Training and Reference Purposes Only . SpectraMax 250 User. -Wide range of applications -Five preferred detection modes -Maximum signal with lowest background The dual monochromator optics allow the widest range of. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SpectraMax® M5e. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Molecular Devices SpectraMax® series microplate readers. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. Cell viability was quantified by measuring the absorbance of the solution at 570 nm using a SpectraMax® M5 microplate reader (Molecular Devices). SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. M3-M5e_manual. TR-FRET assay. View the Molecular Devices website. 6: bioNova científica, s. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The dual monochromator optics allow the widest range of applications to be utilized for bior. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The day before assay, assay buffer was prepared as described in the product technical manual. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. The cells were then treated to induce apoptosis. The PBS solution (10. 96-384. Table 1. Introduction. (The i3x and Paradigm readers require the. Microplate Readers. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Page 28 Chapter 5: Read Modes and Read Types 1. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. • Tel. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Figure 4: Transcreener ADP. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. Call 516-939-0888 to check. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The Spectr. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Fill a clean plate with water. The chamber contains springs that position the cuvette in the proper alignment for a read. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax Lmicroplate luminometer. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. Learn how you can utilize microplate readers to drive landmark discoveries. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Access this by going to Help >. 2. 00 USD. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Molecular Devices | Life Science Applications | Bioanalytical. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. 05% Tween 20 using a Nunc ELISA plate washer. The SpectraMax 190 reader can be integrated with Molecular . 00 USD. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Molecular Devices Spectramax M5 Microplate Reader. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Bottom-reading is usually recommended for cell-based assay with adherent cells. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. Molecular Devices. All associated StakMax® stackers. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. The SpectraMax M5e Multi. Fifteen different automated tests are provided. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Microplate Readers are modular and . The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. SoftMax Pro. The following document is intended to demonstrate setup of this instrument. both cuvette-based and microplate reader applications on the same instrument. 303 unable to cal dark current. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. . The drawing shows a standard microplate of 15 mm height covered with a lid. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. M3-M5e_manual. Between steps, reactions were rinsed 3 times with TBS + 0. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 2. 67. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. 1–0. 2. edu) to be added as a trained user in iLab. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. 0 μL) instead of the enzyme solution was used as the sample blank group. For the Live/Dead assay, they were treated. Has anyone had problems with the built-in computer of the Spectramax ID3. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Page 28 Chapter 5: Read Modes and Read Types 1. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 7°C (gradient heating design). The cuvette must be in place when you read the plate. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. As. This reader's dual monochromators for. es •. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. 0. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. 1. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. #M5) SpectraMax® L Microplate Reader (cat. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Western blotting assays were conducted according to previous studies . With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Download PDF. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Softmax Pro GxP Software 6. AlphaScreen. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Wide range of applications. Selection of Water Constant in PathCheck settings. Please refer to the Obsolete Microplate Reader Models for more information. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orThe optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. B: Selection of Excitation Wavelength. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Anne T. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax® iD5. 1.